When assessing drug metabolism and pharmacokinetics, most researchers instinctively turn to liver microsomes. But what about the gut? The intestinal epithelium is not just a barrier—it’s a metabolic powerhouse that can dramatically alter a drug’s fate before it ever reaches the bloodstream. Ignoring intestinal metabolism means missing critical data on first-pass extraction, oral bioavailability, and drug-drug interactions (DDIs).
That’s where intestine microsomes come in. These ready-to-use subcellular fractions contain high levels of phase I (primarily CYP3A4) and phase II (e.g., UGT) enzymes, enabling you to model the presystemic metabolism of oral drug candidates with unprecedented accuracy.
Why add intestinal microsomes to your ADME toolbox?
- Capture the full picture of first-pass metabolism - Up to 50% of certain drugs are metabolized in the gut wall.
- Detect gut-specific DDIs - CYP3A4 inhibitors/inducers in the intestine can alter exposure even without hepatic effects.
- Improve in vivo predictability - Combine intestinal and hepatic data to refine allometric scaling and PK modeling.
Real-world applications
- Prodrug activation studies - Evaluate conversion efficiency directly in intestinal tissue.
- Metabolite profiling - Identify gut-derived metabolites that may be missed in hepatocyte or liver microsome assays.
- Regional metabolism screening - Compare duodenum, jejunum, or ileum microsomes for targeted oral delivery.
Quality you can trust
Our intestine microsomes are sourced from healthy, ethically procured donor tissues (multiple animal species). Each batch is:
✔ Characterized for CYP450 and UGT enzyme activities
✔ Tested for linearity with incubation time and protein concentration
✔ Provided with detailed donor metadata and QC reports
Empower your ADME and drug metabolism research with high-quality, rigorously validated intestine microsomes to deliver more accurate and reproducible preclinical data. Explore our comprehensive in vitro research solutions to advance your pharmaceutical studies at MileCell.
