MileCell is pleased to announce the release of a side-by-side comparison of Kryogene Cell Freezing Media, designed to help researchers identify the most suitable cryopreservation solution for different cell types, application scenarios, and workflow requirements.

Cryopreservation is an essential step in maintaining cell viability, consistency, and post-thaw performance. However, different research and cell therapy-related workflows may require different formulation features, including DMSO concentration, regulatory support, protein content, safety assessment, packaging format, and applicable cell types.

To support these diverse needs, MileCell offers three Kryogene Cell Freezing Media options: CGT, DMSO Free, and Serum Free.

Three Kryogene Formulations for Different Applications

Kryogene Cell Freezing Media – CGT

Kryogene Cell Freezing Media – CGT is designed for cell therapy-related workflows requiring higher levels of quality and regulatory documentation.

This formulation is serum-free, animal-origin free, protein-free, USP Grade, FDA DMF filed, and clinically applicable. It is available in both bottle and bag formats and contains 10% DMSO.

The CGT formulation is suitable for cryopreservation workflows involving CAR-T cells, CAR-NK cells, expanded NK cells, expanded γδ T cells, MSCs, iPSCs, and other cell therapy products.

With its regulatory filing status and safety assessment support, Kryogene® CGT is intended to help researchers and developers working in cell therapy, translational research, and process development establish more reliable cryopreservation workflows.

Kryogene Cell Freezing Media – DMSO Free

Kryogene Cell Freezing Media – DMSO Free is developed for workflows where avoiding DMSO-related toxicity is a key consideration.

This formulation is serum-free, animal-origin free, DMSO-free, USP Grade, FDA DMF filed, and clinically applicable. It is available in both bottle and bag formats and contains human serum albumin, HSA.

The DMSO Free formulation is recommended for MSCs, neuronal cells, and other DMSO-sensitive cell lines requiring DMSO toxicity avoidance.

For sensitive cell types or workflows where DMSO exposure may affect cell quality or downstream performance, Kryogene DMSO Free provides an alternative cryopreservation option while maintaining strong quality and regulatory support.

Kryogene Cell Freezing Media – Serum Free

Kryogene Cell Freezing Media – Serum Free is a research-grade cryopreservation solution for broad routine cell preservation needs.

This formulation is serum-free, animal-origin free, protein-free, USP Grade, and sterile compliant. It is available in bottle format and contains 10% DMSO.

The Serum Free formulation is suitable for human and animal primary cells, stem cells, immortalized cell lines, and other routine research-use cell types.

For laboratories seeking a ready-to-use, serum-free freezing medium for standard research applications, Kryogene Serum Free offers a practical and accessible option.

Comparison Across 14 Key Parameters

The Kryogene comparison table evaluates the three formulations across 14 key parameters:

Cat. No., packaging format, serum content, animal-derived components, protein content, DMSO concentration, compliance standard, regulatory compliance, product grade, safety assessment, sterility, endotoxin level, recommended application, and applicable cell types.

Across the three formulations, all options are serum-free, animal-origin free, USP Grade, sterile compliant, and have an endotoxin level of < 0.5 EU/mL.

• DMSO concentration: Kryogene CGT and Kryogene Serum Free contain 10% DMSO, while Kryogene DMSO Free is formulated without DMSO.
• Protein content: Kryogene CGT and Kryogene Serum Free are protein-free, while Kryogene DMSO Free contains HSA.
• Regulatory compliance and product grade: Kryogene CGT and Kryogene DMSO Free are FDA DMF filed and clinically applicable, while Kryogene Serum Free is positioned as a research-grade solution.
• Packaging format: Kryogene CGT and Kryogene DMSO Free are available in both bottle and bag formats, while Kryogene Serum Free is available in bottle format.
• Applicable cell types: Each formulation is designed with different application scenarios in mind, from cell therapy-related products and DMSO-sensitive cells to routine primary cell, stem cell, and cell line cryopreservation.

Helping Researchers Choose the Right Cryopreservation Medium

By comparing Kryogene Cell Freezing Media across formulation, quality, regulatory, and application parameters, MileCell aims to provide researchers with a clearer framework for cryopreservation medium selection.

Whether the priority is cell therapy process development, DMSO toxicity avoidance, or routine serum-free cell preservation, the Kryogene® portfolio offers differentiated options to support reliable, reproducible, and workflow-specific cryopreservation outcomes.

Special Offer: Request a Free Cell Freezing Media Sample

To help researchers evaluate the right cryopreservation solution for their workflows, MileCell is now offering one free Cell Freezing Media sample for newly registered MileCell account users.

Register for a MileCell account today and contact our sales team to request your sample.

For sample requests, product ordering, or additional product information, please contact:

sales@milecell-bio.com