2025.11.17
In modern drug discovery, early-stage screening and preclinical evaluation are essential for determining whether a candidate compound is suitable for clinical trials. While animal studies have long played a critical role in pharmacokinetic and toxicological research, regulatory emphasis on ethics and the global shift toward the 3Rs—Replacement, Reduction, andRefinement—are accelerating the adoption of in-vitro systems that are more ethical, efficient, and cost-effective.
Among these in-vitro models, Liver Microsomes and Liver S9 Fractions remain indispensable tools for evaluating drug metabolism. They provide a controlled and reliable platform to study metabolic pathways, intrinsic clearance (CLint), enzyme activity, and potential drug–drug interactions.
Liver microsomes contain a broad spectrum of Phase I metabolic enzymes—most notably the cytochrome P450 (CYP450) family—which play a crucial role in the oxidative, reductive, and hydrolytic metabolism of xenobiotics. By simulating the liver’s primary metabolic processes, microsomes enable researchers to:
* Assess intrinsic clearance and metabolic stability
* Predict ADME characteristics (Absorption, Distribution, Metabolism, Excretion)
* Identify reactive or toxic metabolites
* Evaluate enzyme induction or inhibition, revealing potential drug–drug interactions
As a result, liver microsomes have become a cornerstone in drug metabolism and safety assessment.
Milecell leverages a highly experienced technical team, stringent donor screening procedures, and a robust QC system to deliver high-performance, reproducible, and reliable liver microsomes across multiple species.
Figures 1 and 2 depict stability experiments conducted on Cynomolgus Monkey liver microsome products.
In comparison to liver microsomes from Brand A, the MileCell Bio Lot #CY-V077 liver microsomes exhibit shorter half-lives (t1/2) for three test compounds.
* Large single-batch supply (≥400 vials)
* High metabolic enzyme activity
* Excellent inter-batch consistency
* Strong freeze–thaw stability
* Comprehensive after-sales support
Figures 3 and 4 depict stability experiments conducted on Beagle Dog liver microsome products. Beagle dog microsomes show no significant change in clearance rate after two freeze–thaw cycles, confirming exceptional stability.
Figures 5 and 6 depict stability experiments conducted on CD-1 Mouse liver microsome products. CD-1 mouse microsomes maintain consistent drug clearance within the same batch.
Milecell Subcellular Fractions Product List
Cat Number | Description | Size |
LMSD-100P | Pooled Male SD Rat Liver Microsomes | 0.5mlx 20mg/mL |
LMWS-100P | Pooled Male Wistar Rat Liver Microsomes | 0.5mlx 20mg/mL |
LMWH-100P | Pooled Male Wistar Han Rat Liver Microsomes | 0.5mlx 20mg/mL |
LMBW-100P | Pooled Male Brown Norway Rat Liver Microsomes | 0.5mlx 20mg/mL |
LMCD-100P | Pooled Male CD-1 Mouse Liver Microsomes | 0.5mlx 20mg/mL |
LMCB-100P | Pooled Male C57BL/6 Mouse Liver Microsomes | 0.5mlx 20mg/mL |
LMBN-100P | Pooled Male BALB/c Nude Mice Liver Microsomes | 0.5mlx 20mg/mL |
LMBA-100P | Pooled Male BALB/c Mice Liver Microsomes | 0.5mlx 20mg/mL |
LMBD-100P | Pooled Male Beagle Dog Liver Microsomes | 0.5mlx 20mg/mL |
LMCY-100P | Pooled Male Cynomolgus Monkey Liver Microsomes | 0.5mlx 20mg/mL |
LMRM-100P | Pooled Male Rhesus monkey Liver Microsomes | 0.5mlx 20mg/mL |
LMBM-100P | Pooled Male Bama minipig Liver Microsomes | 0.5mlx 20mg/mL |
LMNR-100P | Pooled Male New Zealand rabbit Liver Microsomes | 0.5mlx 20mg/mL |
LMGP-100P | Pooled Male Guinea Pig Liver Microsomes | 0.5mlx 20mg/mL |
LMMC-100P | Pooled Male Maine Coon Liver Microsomes | 0.5mlx 20mg/mL |
LMFC-100P | Pooled Male Chinese Domestic Cat Liver Microsomes | 0.5mlx 20mg/mL |
LSSD-100P | Pooled Male SD Rat Liver S9 | 0.5mlx 20mg/mL |
LSWS-100P | Pooled Male Wistar Rat Liver S9 | 0.5mlx 20mg/mL |
LSWH-100P | Pooled Male Wistar Han Rat Liver S9 | 0.5mlx 20mg/mL |
LSBW-100P | Pooled Male Brown Norway Rat Liver S9 | 0.5mlx 20mg/mL |
LSCD-100P | Pooled Male CD-1 Mouse Liver S9 | 0.5mlx 20mg/mL |
LSCB-100P | Pooled Male C57BL/6 Mouse Liver S9 | 0.5mlx 20mg/mL |
LSBN-100P | Pooled Male BALB/c Nude Mice Liver S9 | 0.5mlx 20mg/mL |
LSBA-100P | Pooled Male BALB/c Mice Liver S9 | 0.5mlx 20mg/mL |
LSBD-100P | Pooled Male Beagle Dog Liver S9 | 0.5mlx 20mg/mL |
LSRM-100P | Pooled Male Rhesus monkey Liver S9 | 0.5mlx 20mg/mL |
LSBM-100P | Pooled Male Bama minipig Liver S9 | 0.5mlx 20mg/mL |
LSCC-100P | Pooled Male Chinese Domestic Cat Liver S9 | 0.5mlx 20mg/mL |
LSNR-100P | Pooled Male New Zealand rabbit Liver S9 | 0.5mlx 20mg/mL |
LSGP-100P | Pooled Male Guinea Pig Liver S9 | 0.5mlx 20mg/mL |
*Milecell also provides customized preparation of subcellular fractions and Primary hepatocytes from multiple species
Species | Strain |
Rat | SD,Wistar Han,Wistar,Brown Norway... |
Mouse | CD-1,C57BL/6N,C57BL/6J,BALB/c… |
Dog | Beagle |
Monkey | Cynomolgus,Rhesus… |
Minipig | Bama |
Feline | Chinese Domestic Cat, Maine Coon |
Rabbit | New Zealand,Dutch-Belted Rabbit |
Hamster | LVG Hamster |
